However, agrowastes can be used as a substrate in itaconic acid production in conditions of solid state fermentation after optimization. Itaconic acid was first described by baup in 1836 when he discovered it as a product of citric acid distillation. For the presented work, each fermentation broth was filtered and the ph of the solid. Furthermore, new applications of ssf have been suggested for the production of. General and microbiological aspects of solid substrate fermentation. Both acids are excellent platform chemicals with a wide range of applications in different market, such as plastics, coating, or. Itaconic acid also called methylene succinic acid is a white crystalline carboxylic acid obtained by the. Oct 11, 2018 to gain pure, solid itaconic acid, the aqueous solution obtained by back extraction can be fed into a phshift crystallization unit fig. National science council, assignee 2000 method for the production of itaconic acid using aspergillus terreus solid state fermentation. Solidstate fermentation for the production of organic.
The highest itaconic acid yield obtained in a submerged culture batch fermentation was 54. Gluconic acid production by aspergillus terreus dowdells. Us3162582a process for the production of itaconic acid. In this study, the yields of itaconic acid and fumaric acid line 28 were not higher than that of previous study line 85. Itaconic acid ia, an unsaturated dicarboxylic acid, was first recognized during the thermal decomposition of citric acid in 1837. Itaconic acid fermentation by a yeast belonging to the genus.
The main route of gluconic acid production has been by fermentation, mainly using a. Itaconic acid fermentation by yeast 477 spraying kmno4solutions. Production of organic acids by solidstate fermentation. Itaconic acid also called methylene succinic acid is a white crystalline carboxylic acid obtained by the fermentation of carbohydrates. Us5457040a production of itaconic acid by fermentation. Moreover, we can find that the yield of solid fermentation was much lower than that of liquid fermentation, so the benefits of itaconic acid and fumaric acid production by solid fermentation need to be further evaluated.
Historically, itaconic acid was obtained by the distillation of citric acid, but currently it is produced by fermentation. Pdf studies on production of itaconic acid through. Itaconic acid ia production by aspergillus terreus mjl05 strain was investigated in submerged batch fermentation in a stirred bioreactor to determine the effect. The two most important parameters of the fermentation is a high concentration of dissolved oxygen, used directly in the biosynthesis, and keeping ph 4. Among three different fermentation methods, including submerged, surface, and solid state fermentations 12, the most commercial ca has been produced by submerged fermentation of. Aspergillus terreus has been used from the 1940s in the fermentation process, which is. The itaconic acid solubility in the aqueous solution increases drastically around the pk a1 value 3. It provides an overview of current situations for these carboxylic acids and their current production methods. In general, glucose, sucrose and xylose are preferred raw materials for itaconic acid fermentation, which are known to be utilized efficiently by most of the aspergillus sp. Pdf fermentation process for the production of itaconic acid was carried out using jatropha seed cake. Pdf itaconic acid production by novel aspergillus niveus in. Unsaturated solid bond makes a conjugated system with carbonly group.
Ustilago maydis is a promising host for the production of itaconic acid from biomass. Currently, itaconic acid is produced commercially by aspergillus terreus a. World market and biotechnological production of itaconic acid. The fermentation results also indicate that optimal citric acid production conditions were different from optimal itaconic acid production conditions. Itaconic acid was best produced by fungal species than bacterial species.
Identification of the yeast, strain s10 table i showsthe characteristic of this strain. Expired lifetime application number us248293a inventor batti mario alexander. Biomassderived production of itaconic acid as a building. Optimized bioproduction of itaconic and fumaric acids based. Solubilization of animal bonechar by a filamentous fungus. Itaconic acid is a white crystalline unsaturated c5 dicarboxylic acid. It is normally produced by fermentation using fungi, which is more economical compared with thermal decomposition or chemical methods of production lockwood and nelson, 1946. Submerged organic acid fermentation employing agitated, aerated, highquality steel vessels started on the sidelines of penicillin manufacturing.
The name itaconic acid was devised as an anagram of aconitic acid, another derivative of citric acid. Study of itaconic acid production by aspergillus terrus. Itaconic acid solid state fermentation process with peeled sugarcane pressmud as support and starch hydrolysate as carbon source. Pdf biobased itaconic acid market and research trendsis. In particular dicarboxylic acids, like itaconic acid, can be used as monomers for biopolymers. Sorry, we are unable to provide the full text but you may find it at the following locations. Itaconic acid, or methylidenesuccinic acid, is an organic compound. Cassava bagasse was found to be a better substrate than vegetable sponge and sugar cane bagasse.
Both acids are excellent platform chemicals with a wide range of applications in different market, such as plastics, coating, or cosmetics. Solidstate fermentation of itaconic acid by aspergillus. Although some citric acid is produced by solid state fermentation, the estimated 2. Improved itaconic acid production from undetoxified. Production of citric, itaconic, fumaric, and malic acids in. Jul 12, 20 this chapter focuses on recent advances in bioproduction of citric acid, itaconic acid, fumaric acid, and malic acid that are either predominantly or potentially can be produced by filamentous fungi in industrial fermentation. Production of itaconic acid by ustilago maydis from agro. Itaconic acid fermentation by a yeast belonging to the. The market volume of itaconic acid has been estimated to be about 15,000 metric tons per year and is expected to grow if the selling price is reduced. The presence of the conjugated double bond of the methylene group allows polymerization both by addition and condensation. Residual solid material was removed after hydrolysis by centrifugation. Influence of carbon and nitrogen concentration on itaconic. Citric acid production on three cellulosic supports in. Pdf itaconic acid production by novel aspergillus niveus.
Feb, 2019 although some citric acid is produced by solid state fermentation, the estimated 2. Itaconic acid andor salt thereof is produced via aerobic microbial fermentation, for example by means of the species aspergillus terreus or aspergillus itaconicus, of a nutrient medium containing a source of assimilable carbon, such carbon source at least in part comprising an effective amount of glycerol. This study demonstrates the great potential of itaconic acid production using lignocellulose. Method for the production of itaconic acid using aspergillus. Production of citric, itaconic, fumaric, and malic acids. Hydrochloric acid was chosen to allow comparisons with the initial screening experiments and due to the small amounts needed to change the ph to a value less than p k a. Additionally downstream processing using reactive extraction re was described, potentially enabling a more efficient sustainable bioprocess producing ia. Itaconic acid has the stoichiometric formula c 5 h 6 o 4 and a molar weight of. Pdf bioprocessing of sugarcane factory waste to production. The bioproduction of highvalue chemicals such as itaconic and fumaric acids ia and fa, respectively from renewable resources via solid state fermentation ssf represents an alternative to the current bioprocesses of submerged fermentation using refined sugars. Sep 01, 20 in the solid state fermentation process it was introduced as a part of the medium and as a moistening agent. Itaconic acid fermentation using activated charcoal. Synthesis of itaconic acid using for itaconic acid production till now, the m8 strain achieves ustilago maydis. This dicarboxylic acid is a white solid that is soluble in water, ethanol, and acetone.
Optimized bioproduction of itaconic and fumaric acids based on. Fermentative itaconic acid production hilaris publishing srl. Itaconic acid fermentation using activated charcoaltreated. Solid state fermentation for the production of organic acids. Nov 18, 20 itaconic acid is a valuable platform compound for the production of bio. Even a temporary cessation of aeration such as during the manual. Citric acid production on three cellulosic supports in solid. Itaconic acid ia has a high potential to be used as a bio. United states united states werpy t, petersen g 2004 top value added chemicals from biomass. This article shows the recent developments on processes and products developed for the value addition of sugarcane bagasse through the biotechnological. Itaconic acid ia is a biobased chemical with great potential for the. Itaconic acid ia is one of the most important organic acids that can be. Solidstate fermentation ssf has emerged in the last decades as a promising industrial process for several products, especially using.
Six strains of aspergillus niger were screened in liquid medium and strain lpb 21 was selected for further studies on three different agroindustrial wastesresidues for production of citric acid by solid state fermentation. The method to produce itaconic acid is a solid state fermentation method. Itaconic acid is produced solely by batch submerged fungal fermentation. The conditions used here are identical to those used in example 1, except that the glucose10% wv in medium a was replaced by 33% wv starch hydrolysate which contained 30% wv total sugar. Itaconic acid a versatile building block for renewable. By the optimization of media components and process parameters, a. Enhanced itaconic acid production in aspergillus niger using. Itaconic acid 2methylenesuccinic acid, 1propene23dicarboxylic acid is an unsaturated, weak dicarboxylic acid pka 3. A possible substrate for ia production with two strains of a. Us6171831b1 method for the production of itaconic acid using. Moreover, itaconic acid was produced from di erent sugar monomers based on renewable feedstocks and the.
In order to promote the biobased economy, the usdepartment of energy doe assigned a top12 of platform chemicals, which include numerous of organic acids. It exists as white to light beige crystals with a density of 1. Sustainable carbon sources for microbial organic acid production. Pdf microbiological features of solid state fermentation.
Valencia orange citrus sinensis peel was employed in this work as raw material for the production of citric acid ca by solid state fermentation ssf of aspergillus niger cect2090 atcc 9142. Optimized bioproduction of itaconic and fumaric acids. The same organism under different fermentation conditions and with a higher operating ph elaborates secondary metabolites, including lovastatin bizukojc and. Our opinion is that the itaconic acid production using a. Microbiological features of solid state fermentation and its applications an overviewsolid state fermentation bashir sajo mienda solid state fermentationin contrast to submerged liquid state fermentation, solid state fermentation ssf is the growth andor cultivation of micro organisms under controlled conditions in the absence of free. Pdf production of itaconic acid by ustlago maydis from. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed. Among three different fermentation methods, including submerged, surface, and solid state fermentations 12, the most commercial ca has been produced by submerged fermentation of aspergillus. Improved itaconic acid production from undetoxified enzymatic.
Production of itaconic acid by ustilago maydis from agro wastes in solid state fermentation. Microbiological features of solid state fermentation and its applications an overviewsolid state fermentation. Production of itaconic acid through microbiological. Aug 16, 2010 another carboxylic acid, itaconic acid methylenesuccinic acid, is biosynthesized by a. Solidstate fermentation for the production of organic acids. This study focused on the production of itaconic acid from a lignocellulosebased carbon source by a. Metabolic engineering of saccharomyces cerevisiae for.
Enhanced itaconic acid production in aspergillus niger. The conditions used here are identical to those used in example 1, except that the glucose10% wv in medium a was replaced by. Us3162582a us248293a us24829362a us3162582a us 3162582 a us3162582 a us 3162582a us 248293 a us248293 a us 248293a us 24829362 a us24829362 a us 24829362a us 3162582 a us3162582 a us 3162582a authority us united states prior art keywords itaconic acid fermentation acid medium acids prior art date 19621231 legal status the legal status is an assumption and is. Itaconic acid is exclusively produced this way bafana and pandey 2018. Pdf production of itaconic acid by ustlago maydis from agro. Production of itaconic acid through microbiological fermentation of inexpensive materials. Fungal fermentation of lignocellulosic biomass for itaconic and. Study of itaconic acid production by aspergillus terrus mjl05 strain. On the other hand, it was reported that addition of other carbohydrates such as glucose or sucrose reduced the activity of some lignocellulolytic enzymes emtiazi et al. Because of its specific favorable properties and the unique structure, itaconic acid is used worldwide as monomer or comonomer in manufacturing plastics, resins etc 1,2. Pdf optimized bioproduction of itaconic and fumaric acids based. Optimum fermentation medium and ph for itaconic acid production were found to be 3.
However, there was no comprehensive study on using latest technologies for increasing the productivity at industrial level and it was not properly established. Itaconic acid ia production by aspergillus terreus mjl05 strain was investigated in submerged batch fermen tation in a stirred bioreactor to determine the. Isolation and identification of the acid from these characteristics, strain. Jul 19, 20 solidstate fermentation ssf or solid substrate fermentation has been defined as the fermentation process occurring in the absence or nearabsence of free water. Some recent studies demonstrated that the application of the biore.
114 229 1077 123 128 77 831 347 515 937 227 614 544 289 334 756 1473 1388 208 31 68 159 946 758 130 322 855 386 570 1079 448 1404 800