Isolation of efficient phosphate solubilizing bacteria. Among the whole microbial population in soil p, solubilizing bacteria comprise 150% and p solubilizing fungi 0. Phosphate solubilizing bacteria psb are being used as biofertilizer since 1950s kudashev, 1956. Gluconic acid seems to be the most frequent agent of mineral phosphate solubilization. In general among the whole microbial population in soil p solubilizing bacteria constitute 1 50% and p solubilizing fungi 0. Of 31 isolated psb strains, two efficient strains, identified as pantoea agglomerans psb1 and burkholderia anthina psb2, were employed in further studies. These are generally referred as phosphate solubilizing bacteria psb. Phosphate solubilizing bacteria bioline international. It showed that all of the tested isolates were able to dissolve the inorganic phosphate of ca 3 po 4 2 within the media of pikovskaya. Molecular characterization of phosphate solubilizing bacteria. Molecular characterization of phosphate solubilizing.
Introduction rhizosphere is the soil zone surrounding the plant roots while rhizoplane is directly in contact with the roots kennedy, 2005. Pdf survival of phosphatesolubilizing bacteria against. Strains from the genera pseudomonas, bacillus and rhizobium are among the. Mar 01, 2017 phosphate solubilizing bacteria psb play an important role in biogeochemical phosphorus cycling in both terrestrial and aquatic environments. The phosphate solubilizing bacteria were present in all the soil samples ankober, keyt, mehalmeda, and molale. Highly efficient phosphatesolubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. Pot1, can solubilize tricalcium phosphate ca 3 po 4 2 at a rate of 956 mg l. It is the least mobile element in plant and soil contrary to other. Diameter of halo zone was measured after 42 hours, up to 15 days. This study found bacteria that solubilized phosphate at very high rates. We report the establishment of psb strains serratia marcescens eb 67 and pseudomonas sp. The production of organic acids by phosphate solubilizing bacteria has been well documented.
Phosphatesolubilizing bacteria from safflower rhizosphere. Safflower is a cash crop that is a source of vegetable oils, food coloring and flavoring agents. Based on phosphate solubilizing indices after three days and quantification of tcp solubilization three strains viz. This evidence points to the advantage of the mixed inoculations of pgpr strains comprising phosphate solubilizing bacteria. Liquid biofertiliseruseful in all crops including legumes.
Mostly, one gram of fertile soil contains 10 1 to 10 10 bacteria, and their live weight may exceed 2,000 kg ha. Isolation and characterization of phosphatesolubilizing bacteria. Cdb 35, screened using rock phosphate rp buffered medium in the rhizosphere and their. Phosphate solubilizing bacteria psb can convert insoluble rhizosphere phosphorus into forms that are absorbable by plants and thus enhance the growth of plants. To reduce the addition of mineral phosphorus to agricultural soils, research in naturally occurring phosphate solubilizing microorganisms has been conducted for. Ros reactive oxygen species, acc 1aminocyclopropane1carboxylate, nh 3 ammonia, hcn hydrogen cyanate, iaa indole3acetic acid, p phosphate 3 biotech 2015 5. Five bacterial strains with phosphate solubilizing ability and other plant growth promoting traits increased the plant biomass 2040% by paper towel method. Characteristics of inorganic phosphatesolubilizing bacteria.
Utilization of phosphate solubilizing bacteria as biofertilizers instead of synthetic chemicals is known to improve plant growth through the supply of plant nutrients, and may help to sustain environmental health and soil productivity. Pdf effect of rhizobium and phosphate solubilizing. Combined use of pr and phosphorous solubilizing bacteria psb in different. Fungi are important components of soil microbiota typically constituting more of the soil biomass than bacteria, depending on soil depth and nutrient conditions. Feb 01, 2016 16 phosphate solubilizing bacterial strains have been isolated from the rhizosphere of l. Almost one hundred strains were grown on an lb broth initially for robust. Pdf plants acquire phosphorus from soil solution as phosphate anion. Phosphate solubilizing bacteria psb have the ability to solubilize insoluble phosphorus p and release soluble p.
Isolation and classification of nitrogen fixing and phosphate. Assessment of their effect on growth promotion and phosphorous uptake of mung bean vigna radiata l. P4, p9 and p10 were selected as potent phosphate solubilizers. Isolation, screening and characterization of phosphate. The ability of a few soil microorganisms to convert insoluble forms of phosphorus to an accessible form is an important trait in plant growthpromoting bacteria for. Phosphate solubilizing fungi were found in most soils tested. Phosphatesolubilizing bacteria psb have the ability to solubilize insoluble phosphorus p and release soluble p. Various phosphate sources were investigated, and ca 3 po 4 2 was identified as the effective phosphate source.
Phosphate solubilizing bacteria psb english youtube. Also, 2ketogluconic acid is another organic acid identified in strains with phosphate solubilizing ability table 1. Soil is a natural basal media for microbial growth. Identification of phosphatesolubilizing microorganisms and. Effect of phosphate solubilizing fungi on growth and nutrient. Isolation and characterization of phosphate solubilizing. Isolation of phosphate solubilizing bacteria from the.
Phosphate solubilizing microorganisms psm, phosphate solubilizing fungi psf, phosphates olubilizing bacteria psb, acid production, phosphorus p, p solubilization, hplc, p immobilization created date. Organic acids production and phosphate solubilization by. An efficient protocol was developed for qualitative screening of phosphate solubilizing bacteria, based upon visual observation. All the bacterial isolates solubilized significantly p. Pdf survival of phosphatesolubilizing bacteria against dna. Many bacteria and fungi are able to improve plant growth by solubilizing inorganic and organic phosphates in the soil 9,10. Pdf phosphate solubilizing bacteria and their role in plant growth.
Isolation and application of salttolerant or halophilic psb will facilitate the development of salinealkali soilbased agriculture. Comparative efficiency of five potash and phosphate. They have been reported to help in plant growth through smooth provision of p to the plants dey et al. Phosphate solubilizing bacteria after nitrogen, phosphorus p is the second essential macronutrient for plant growth and development. Different strains of phosphatesolubilizing bacteria psb were isolated from the rhizosphere of different plants of lahore district, pakistan. Microbial phosphorus solubilization and its potential for. Among the chickpea genotypes, ig593 performed better in respect of symbiotic parameters including nodule number, nodule fresh weight, nodule dry. May 07, 2016 phosphate, and rock phosphate roy choudhury and kaushik, 1989. Isolation and classification of nitrogen fixing and. Phosphate solubilizing activity of some bacterial strains.
The effects of different insoluble phosphate media on. Comparative efficiency of five potash and phosphate solubilizing bacteria and their key enzymes useful for enhancing and improvement of soil fertility sajidmohammad a saiyad, dr. Our results indicate that, by using our formulation containing bromophenol blue, it is possible to quickly screen on a qualitative basis the phosphate solubilizing bacteria. Phosphorus solubilizing bacteria and biochar improve maize. Growth promotion of maize by phosphate solubilizing.
The highest amount of solubilization was recorded for the bacterial isolate meh305 39 mg followed by meh303 34 mg, meh101 27 mg, jim41. Initially, 50 isolates were isolated on the basis of zone of clearance around their. The most efficient of the bacteria presented in this paper, pantoea sp. Generally, substantial amount of phosphorus p occurs in soil ranging from 400 to 1,200 mgkg of soil, either in mineral. A wide range of soil fungi are reported to solubilize insoluble phosphorous. Phosphate solubilising bacteria, saline soil, tricalcium phosphate, plant growth promoting traits introduction. But bacteria form the largest group which bring about mineralization of organic phosphorus compounds and are known as phosphate solubilizing bacteria asea, 1988. The goal of this research lies in the identification of beneficial bacteria capable of fixing nitrogen and solubilizing phosphate. Identification and characterization of the phosphate. They are considered as one of the key groups of soil micro flora involved in p cycling. Bano, rhizobium and phosphate solubilizing bacteria improve the yield and phosphorus uptake in wheat triticum aestivum l. A small number of studies investigated on the occurrence of phosphate solubilizing bacteria in the marine environment and fresh water environment like lake ecosystem. Characterization indigenous phosphate solubilizing of. As a group, the fungi were superior to bacteria in solubilizing both freshly precipitated calcium phosphate and idaho rock phosphate.
Several researchers have reported that inoculation with phosphatesolubilizing bacteria result in improved growth, yield, and p uptake in several crops hameeda et al. Isolation and identification of phosphate solubilizing bacteria psb in the present study, the collected soil samples were evaluated in vitro for p solubilising bacteria in pikovskayas pkv plates supplemented with 1. Phosphate solubilizing bacteria psbs were isolated from different plant rhizosphere soils of various agroeco logical regions of india. Phosphate solubilizing bacteria psb, available p, bacillus, phosphorus, cotton. Psb could convert these insoluble phosphates into available forms for plant via acidification, chelation, exchange reactions, and production of gluconic isolation and characterization of phosphate solubilizing bacteria.
Microbial phosphorus solubilization and its potential for use. Qualitative analysis of the phosphate solubilized by various groups correlated well with. The use of phosphate solubilizing bacteria as inoculants simultaneously increases p uptake by the plant and crop yield. Pdf phosphate solubilizing bacteria and their role in plant. Inorganic phosphate solubilizing bacteria ipb are an important microbial class in sediments, playing an essential role in the phosphorus cycle of. Dec, 2014 table 2 3 description of the source and the numbers of putative nitrogen fxing bacteria and phosphate solubilizing bacteria 50 table 2 4 enumeration of putativen fixing ang p solubilizing bacteria isolated from. There are considerable populations of phosphate solubilizing bacteria in soil and in plant rhizosphere. Phosphate solubilizing bacteria psb function in soil phosphorus cycle, increasing the bioavailability of soil phosphorus for plants.
The goal of this project is to test isolated phosphate solubilizing bacteria psb from mendocino ecological staircase soil samples and screen them for their ability to solubilize different insoluble phosphates in growth media. Phosphate solubilizing microorganisms have considerable synergistic e. They are gaining importance because of ecofriendly, nonhazardous, and nontoxic nature kannaiyan et al. With the help of nitrogen fixing microbes, plants obtain ammonia as nitrogen source from soils. The aim of this work was to isolate phosphate solubilizing bacteria psb and assess their effect on the growth of mung bean vigna radiata l. Isolation and characterization of a phosphatesolubilizing. However, there is a limit on the amount of phosphate which can be added to the environment due to the issue of eutrophication. Use of the phosphatesolubilizing bacterial preparation. Release of p by psb from insoluble and fixed adsorbed forms is an import aspect regarding p availability in soils.
Phosphate solvent bacteria are a group of soil bacteria that are capable of dissolving p fixed by soil minerals and liberating porganic so that they can be converted into available forms for plants to absorb susilowati and syekhfani, 2014. Effect of phosphate solubilising bacteria bacillus psb24 on. The qualitative as well as quantitative analysis of phosphate solubilizing activity of the selected isolates were conducted by plate screening method and broth culture method, respectively. Phosphate solubilizing bacteria from subtropical soil and their. An efficient method for qualitative screening of phosphate. The use of efficient psm phosphate solubilizing microorganisms. Ps bacteria were inoculated into 50 ml nbrip broth. Phosphatesolubilizing bacteriaassisted phytoremediation of.
Phosphate solubilization and mobilization in soil through. A moderately halophilic bacterium was isolated from the sediment of daqiao saltern on the eastern coast of china, which also. However, they were found to differ both in number, as estimated by colony forming units per gram of soil, and efficiency as observed from clear zone diameter around their colonies. Several bacterial and fungal species with varied potentials to solubilize inorganic phosphates, also known as phosphate solubilizing microorganisms, have been. Psms are ubiquitous, and their figures differ from soil. The main purpose of this study was to identify new phosphate solubilizing bacteria isolated. Pdf phosphate solubilizing bacteria and their role in. The effects of different insoluble phosphate media on phosphate solubilizing bacteria from mendocino terrace soil danielle naiman1, alex aaring2, shi wang2, bryson cwick2, romy chakraborty2 2015 transfertoexcellence research experiences for undergraduates program tte reu program 1santa monica college, 2lawrence berkeley national laboratory. The detailed information about materials used and methods adopted for the present research work has been elaborated in this chapter. Efficiency of p fertilization in acid sulphate soil for rice cultivation can be improved by phosphate solubilizing bacteria psb application. Phosphate solubilizing bacteria psb can convert the insoluble phosphates into plantavailable forms and may have the potential for use in.
Selection and evaluation of phosphatesolubilizing bacteria. The ability of phosphate solubilizing bacteria to quantitative dissolve phosphate and the ph of the liquid medium with various phosphorus sources after 5 days incubation were measured using spectrophotometer. In this study, highthroughput isolation hti was used to identify n fixing andor p solubilizing bacteria. Pdf phosphorous is one of the most abundant metallic element found in.
Out of 50 bacterial isolates, 10 isolates having larger halo zones were selected for further study. Screening of phosphate solubilizing microorganism in solid medium the ability of the isolated microorganisms to solubilize insoluble phosphate was calculated by. Isolation and characterization of phosphatesolubilizing. Effect of phosphate solubilizing fungi on growth and. Plant roots exuded the organic contents in the rhizosphere and subsequently increased. Highly efficient phosphate solubilizing bacteria can be used to increase phosphorus in the plant rhizosphere. There are strong evidences that soil bacteria are capable of transforming soil p to the forms available to plant. Phosphate solubilizing bacteria generally outnumber p solubilizing fungi by 2150 folds. An alternative approach for the use of phosphate solubilizing bacteria as microbial inoculants is the use of mixed cultures or coinoculation with other microorganisms. Qualitative estimation of all the suspected phosphate solubilizing bacteria were screened by inoculating and growing on pvk medium and nbrip medium with bromophenol blue. Screening of phosphate solubilizing microorganism in solid medium the ability of the isolated microorganisms to solubilize insoluble phosphate was calculated by solubilization efficiency e 9. They have been reported to help in plant growth through smooth provision of p to the plants dey.
Pdf the use of phosphate solubilizing bacteria as inoculants simultaneously increases p uptake by the plant and crop yield. A field experiment was carried out during the rabi season of 200405 to find out the effect of rhizobium and phosphate solubilizing bacterial psb inoculants on symbiotic traits, nodule leghemoglobin, and yield of five elite genotypes of chickpea. Effects of phosphate solubilizing bacteria on the growth. The main purpose of this study was to identify new phosphate solubilizing bacteria. Phosphate solubilizing bacteria psb have the potential to enhance phosphate induced immobilization of metals to remediate contaminated soil.
Phosphatesolubilizing bacteriaassisted phytoremediation. Wilczek buddhi charana walpola 1, and minho yoon1 the aim of this work was to isolate phosphate solubilizing bacteria psb and assess their effect on the growth of mung bean. Growth promotion of maize by phosphate solubilizing bacteria. Factors governing the phosphate solubilizing activity of the strains included carbon and nitrogen sources, initial ph, and fermentation time. Among the bacterial genera with this capacity are pseudomonas, bacillus, rhizobium, burkholderia, achromobacter, agrobacterium, microccocus, aereobacter, flavobacterium and erwinia. Phosphate solubilizing bacteria will dissolve pabsorbed by. Identification of phosphatesolubilizing microorganisms. Fungi also retained this ability over many sub culturing transfers. Pdf, phosphatesolubilizing bacterial activity and their. Population of phosphate solubilizing bacteria in the liquid organic fertilizer created from palm oil bunches and pineapple rhizome.
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